Composite

Part:BBa_K1913030:Design

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)


Synthetic plac-FixK2 hybrid promoter+RBS with mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
    Illegal AgeI site found at 723
    Illegal AgeI site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to test synthetic plac-FixK2 hybrid promoter,we put reporter mRFP under this promoter and co-transformed with light sensor BBa_K1913034. And mRFP fluorescence value was tested so that the intensity of transcription of this promoter could be quantified.


Source

Genomic sequence of Bradyrhizobium japonicum, Genomic sequence of E.coli, iGEM part registry.

References